Product

Avian Influenza Virus Universal-H7 Subtype Antigen Duplex Test Card (Colloidal Gold Method)

The H7 subtype of avian influenza virus attracts particular attention because strains such as H7N9 can infect humans, and its highly pathogenic variants often cause high mortality…

Key parameters

SKUFZCP009
MethodColloidal Gold Immunochromatography
SpecificationStrip
Storage2–30 °C, dry, protected from light
Shelf life24 months

Description

Target and Hazard: The H7 subtype of avian influenza virus attracts particular attention because strains such as H7N9 can infect humans, and its highly pathogenic variants often cause high mortality in poultry; highly pathogenic avian influenza is a Class I animal disease in China. The virus replicates mainly in the respiratory and digestive tracts of poultry and spreads through secretions, faeces and contaminated materials, with live-bird trade and transport being important routes of dissemination. Once a flock is infected, both egg production and survival fall and there is a public-health risk of human infection, so rapid on-site identification of H7 has a double significance.

Significance of Testing: The duplex card carries two test lines on a single strip: the universal line recognises the type-specific antigen shared by all type A avian influenza viruses and quickly indicates whether avian influenza virus is present, while the H7 line identifies the H7 subtype. Reading both lines completes the on-site question 'is it avian influenza, and is it H7?' within 15 minutes. It is intended for outbreak investigation on poultry farms and in live-bird markets, quarantine testing of poultry movements and environmental screening of flocks linked to suspected human cases. A positive result must be reported and laboratory-confirmed in accordance with national regulations.

Principle: Colloidal gold immunochromatographic assay (double-antibody sandwich): the type-specific antigen and the H7 subtype antigen in the sample each bind their corresponding gold-labelled antibody; on migrating to the universal test line (T1) and the H7-subtype test line (T2) they are captured by immobilised antibodies and produce visible lines, with the control line (C) confirming reagent and procedure validity.

Performance and Applications: No instrument required; visual reading in 10–15 minutes; samples: tracheal/cloacal swabs, tissue homogenate; store and ship at 2–30 °C, shelf life 24 months. Suitable for rapid on-site screening on poultry farms, in live-bird markets, slaughterhouses, primary veterinary stations and at border quarantine, and can be used alongside the universal–H5 subtype duplex card. Method: colloidal gold immunochromatographic assay (double-antibody sandwich) | Samples: tracheal/cloacal swabs, tissue homogenate | Time to result: 10–15 min | Reading: visual assessment of T1 (universal)/T2 (H7 subtype)/C lines | Format: tests/box (as stated on the label) | Storage: 2–30 °C, cool and dry; shelf life 24 months | Note: sensitivity, specificity, limit of detection, intra-/inter-assay CV and agreement with RT-PCR/virus isolation are subject to our internal QC report.

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