Classical Swine Fever Virus (CSFV) ELISA Antibody Detection Kit (Indirect Method)

Target and Hazard: Classical swine fever is caused by classical swine fever virus (CSFV). It is a Class II animal disease in China and a priority disease in swine immunisation programmes; under the national compulsory vaccination guidelines, provinces may include classical swine fever in their own compulsory vaccination scope according to local epidemiology. The virus infects domestic pigs and wild boar and spreads through direct contact and contaminated feed, water, personnel or equipment, as well as across the placenta. Affected pigs develop high fever, anorexia, constipation followed by diarrhoea, and haemorrhages in the skin and internal organs, with high mortality in acute cases; chronically and subclinically infected pigs shed virus for long periods and act as a continuous source of infection, seriously affecting sow reproductive performance and piglet survival.

Significance of Testing: The kit detects CSFV-specific antibodies in serum. Its indirect format offers high throughput at low cost, making it suitable for large-scale serosurveys of vaccination-induced antibody levels and herd immune-qualification assessment, for monitoring antibody kinetics after vaccination and for decisions on schedule adjustment or booster vaccination, and for tracing infection history in epidemiological investigations. Suspected acute cases should be confirmed by antigen or nucleic acid testing.

Principle: Indirect ELISA: microplates are coated with CSFV-specific antigen; antibodies in the sample bind to it and are recognised by an enzyme-labelled anti-pig IgG secondary antibody. Substrate is added and colour intensity is directly proportional to antibody level; because an anti-pig IgG conjugate is used, the assay applies to porcine serum and plasma.

Performance and Applications: 96-well format, approximately 40–44 samples per plate including controls; total assay time approximately 2–2.5 hours; read at 450 nm for objective results suited to high-throughput screening; storage at 2–8 °C. Intended for routine vaccination monitoring on large-scale pig farms and for herd serosurveys by animal disease control institutions and third-party laboratories. Method: indirect ELISA (CSFV-specific antigen coated, enzyme-labelled anti-pig IgG conjugate) | Samples: porcine serum/plasma | Assay time: ~2–2.5 h | Reading: microplate reader at 450 nm, interpreted from the S/P or OD value | Format: 96-well plate, ~40–44 samples per plate | Storage: 2–8 °C, protected from light; shelf life 12 months | Note: sensitivity, specificity, limit of detection, intra-/inter-assay CV and agreement with reference methods are subject to our internal QC report.